Various library types are available for Illumina NovaSeq and MiSeq sequencing, including:
- DNA libraries (shotgun and PCR-free) for sequencing complete genomes (and metagenomes)
- RNA libraries (mRNA, lncRNA, small RNA)
- Metabarcoding (16S/18S/ITS/Other targets)
- DNA libraries for identification of methylation sites
- Enriched libraries for regions of interest
- Exome capture
- Customized panels
- Methylation sites
- RNA-Exome
- Amplicon libraries
- Single cell libraries
- GBS libraries (RAD, ddRAD)
- ATAC-Seq libraries
- HiC libraries
- ChIP-Seq libraries
Most library preparation pipelines have been automated to increase robustness and cost efficiency.
Different sequence lengths (between 100bp and 300bp) are available for different applications. Libraries can be sequenced in single-end or paired-end format.
FASTQ files are the usual deliverables for the Illumina sequencing, but it is possible to generate BAM files for Human, Mouse and Rat species. The sequencing data can be downloaded directly from Nanuq.
To find out the amount of data generated by each instrument and to determine the appropriate sequencing options for your project, please contact the Client Management Office.
