Infinium genotyping (Illumina®)
- Analysis of between 3,000 and over 5 million loci (SNPs and indels) in parallel
- Custom genotyping to study specific regions of interest in any species
- Commercial biochips with standard content
Applications
- Genome-Wide Association Studies (GWAS) to map genetic variation among populations in order to identify phenotype-associated variants
- SNP discovery validation
- Copy Number Variation (CNV) analyses
- Cytogenetic studies
- Homozygosity mapping
Description
The Infinium (high-density) test is designed for genotyping, from customized studies of targeted regions to genome-wide association studies, CNV (Copy Number Variation) analyses and cytogenetic studies. Only bi-allelic markers such as SNPs (Single Nucleotide Polymorphisms) and indels (insertion/deletion polymorphisms) can be genotyped.
The reaction consists of an initial amplification of the entire genome, followed by fragmentation. The product is then hybridized on a planar silica slide whose microwells contain beads coated with specific primers serving as addresses. Each type of bead is represented numerous times to increase precision. The hybridized product is then extended with labeled nucleotides and the signal detected with a scanner. The typical success rate is over 98%.
Visit the Illumina website for more details.
Produits disponibles
BeadChips with standard or customized content (iSelect Custom Genotyping) are available. For custom content biochips, a minimum of 1,152 samples is required.
Several factors influence the conversion rate of Infinium custom content microarrays, including Illumina’s rigorous manufacturing quality control criteria, the nature of the SNP and indel sequence selected, and the criteria used during analysis. To maximize the chances of success, it is recommended to select validated markers and/or markers with a high design score.