Illumina Technology
User Guide
The instructions in this guide must be followed carefully to achieve the best possible results and avoid delays in processing your request.
Any samples that do not comply with the recommendations may be rejected without compensation.
Note that delays in the processing of libraries will vary depending on the size of the project. It is recommended to contact the Client Management Office for information regarding processing time.
Quick search below
- Starting Material
- Requirements – Quality and Quantity
- Required Containers and Sample Identification
- Service Request Form and Sample Submission
- Sample Shipment Preparation
- Transmission of results
Starting Material
Total RNA is used as the starting material for all protocols.
Practical advice and good laboratory practices
- Samples should be resuspended in commercial RNase‑free water or in the elution buffer provided in commercial kits.
- Samples should be treated with DNase to eliminate the possibility of DNA contamination.
- If Trizol is used to extract RNA samples, a final cleanup should be performed (e.g., using a column‑based cleanup kit) before submitting the samples.
- DEPC‑treated water should be avoided. Residual DEPC degradation products can inhibit subsequent reactions.
- RNA should not be resuspended in solutions containing detergents (e.g., SDS), as these can interfere with enzymatic reactions.
Requirements – Quality and Quantity
| Library preparation type | Quantity | Concentration | Volume | Quality* |
|---|---|---|---|---|
| Poly(A) Selection | ≥300 ng | ≥10 ng/µL | ≥30 µL | RIN ≥6.5 Ratio 260:280 1.8-2.1 Ratio 260:230 1.8-2.2 |
| Poly(A) Selection + globin Depletion | ≥300 ng | ≥10 ng/µL | ≥30 µL | RIN ≥6.5 Ratio 260:280 1.8-2.1 Ratio 260:230 1.8-2.2 |
| Low Input Poly-(A) Selection (non directional, for quantity lower than 25ng) | ≥0.5 ng | ≥0.06 ng/µL | ≥10 µL | RIN ≥6.5 Ratio 260:280 1.8-2.1 Ratio 260:230 1.8-2.2 |
| Bacterial rRNA Depletion and Meta-transcriptomic rRNA Depletion (bacterial rRNA + host) | ≥200 ng | ≥10 ng/µL | ≥20 µL | RIN ≥6.5 Ratio 260:280 1.8-2.1 Ratio 260:230 1.8-2.2 |
| rRNA Depletion from different species (Human, mouse, rat, plant, fish, etc.) | ≥320 ng | ≥32 ng/µL | ≥10 µL | RIN ≥6.5 Ratio 260:280 1.8-2.1 Ratio 260:230 1.8-2.2 |
| Small RNA profiling (<200 nt) and/or miRNA (21–25 nt) | ≥750 ng | ≥63 ng/µL | ≥12 µL | RIN ≥6.5 Ratio 260:280 1.8-2.1 Ratio 260:230 1.8-2.2 |
| Total RNA | ≥50 ng | ≥10 ng/µL | ≥5 µL | RIN ≥6.5 Ratio 260:280 1.8-2.1 Ratio 260:230 1.8-2.2 |
| RNA exome | ≥20 ng | ≥1.2 ng/µL | ≥12 µL | RIN ≥6.5 Ratio 260:280 1.8-2.1 Ratio 260:230 1.8-2.2 |
| RNA exome (FFPE RNA) | ≥30 ng | ≥2.4 ng/µL | ≥12 µL | DV200 ≥36.5 |
* RIN = RNA Integrity Number
– The use of out‑of‑specification samples may limit the detection of low‑expressed genes, compromising the sensitivity and representativeness of the analysis.
– The RIN value for rRNA‑depletion protocols may be lower than 6.5. Contact a member of the Client Management Office to discuss this.
– The platform’s ability to generate small RNA libraries may vary depending on sample characteristics and experimental conditions. Contact a member of the Client Management Office to discuss this.
Replacement samples are accepted and must meet the same criteria as the original samples. Partial replacements (‘top‑ups’) are not accepted.
Required Containers and Sample Identification
- It is recommended to send samples in low‑binding tubes.
- The name written on the tube must be unique, legible, short, and identical to the one indicated on the submission form.
| Accepted Formats | Unaccepted formats |
|---|---|
| 1.5mL Tubes 2mL Tubes | Strip Tubes 0.2mL Tubes 0.5mL Tubes 2D Tubes All plate formats |
Service Request Form and Sample Submission
Click on request a service and submit samples to view the instructions.
Sample Shipment Preparation
Click on samples shipment preparation to view the instructions.
Transmission of Results
A message is sent to all contact persons listed in the Nanuq project as soon as the results of the initial sample quality control are available. The same applies when the library quality control results become available
An automated notification from Nanuq will be sent to the principal investigator and project contacts once sequencing data are available.
Sequencing results are directly accessible via the Nanuq web application.
For more information on downloading data and available files, please refer to the Frequently Asked Questions page.
Version 2.0_2026-04-21