Instructions
All service request and sample submissions must be created on Nanuq before sending samples. A user account and an official quote (except for Sanger and PlasmiQ services) are required. To obtain an account and/or a quote, contact the Client Management Office.
Lab work will only begin once all documentation has been submitted. Incomplete documentation will result in project processing delays.
Service Request
Extraction, library preparation, sequencing, genotyping services
For any service requests, contact the Client Management Office to determine the best options for your project and obtain a quote.
Then, follow the steps below to create a request form and submit samples:
1. Log in to Nanuq.
2. Click “Add New Request” in the “Requests” section and follow the instructions.
3. Click “Next” to move to the next page of the request.
To request the return of samples upon project closure, indicate this under the “Sample Information” tab and provide the requested information.
| Sample Type | Biological samples for extraction | RNA | gDNA | Libraries | Sanger Sequencing | Microsatellite Genotyping – Labeled PCR | Microsatellite Genotyping – gDNA | Whole plasmid sequencing and long PCR |
|---|---|---|---|---|---|---|---|---|
| Option to return samples at project closure | ✔️ | ✔️ | ✔️ | ✖️ | ✖️ | ✖️ | ✔️ | ✖️ |
| Sample retention period, after sequencing, before destruction | 3 months | 3 months | 3 months | 3 months | 2 weeks | 1 month | 3 months | 2 weeks |
4. Click “Submit”.
5. The principal investigator will receive an email inviting them to review and sign the application. They must open the application link provided in the email and click “Sign and Submit” after reviewing it.
An unsubmitted/unsigned request cannot be approved by the Client Management Office and will not be processed.
Practical advices
- The “New Request” option should not be used to complete an existing request.
- Do not use the browser’s “Back” button to go back. Use the page options in the left-hand menu to navigate through the form.
- Click “Next” to move to the next page of the request.
- The information can be saved at any time by clicking “Save and continue later.” Drafts can be accessed via the ‘Requests’ option in the “Requests” section.
- The request remains a draft until it is submitted. To edit a request with draft status, click on “Edit” in the left-hand men.
Sanger sequencing service
1. Download and complete the Sanger Sequencing Service Request Form.
2. Email this completed and signed form to the Client Management Office along with:
a. A copy of the ethics committee approval forms if the submitted samples were obtained from human subjects.
b. A purchase order, if this is the chosen payment method.
Within 24 hours, an automated email from Nanuq is sent:
c. When a new user creates an account, a username and temporary password are assigned, allowing online sample submissions to Nanuq
d. When a new project is opened.
PCR amplification and Sanger sequencing
For any service requests, contact the Client Management Office to determine the best options for your project and obtain a quote.
Then, follow the steps below to open an account and submit samples:
1. Download and complete the Sanger Sequencing Service Request Form.
2. Email this completed and signed form to the Client Management Office along with:
a. The Sample Submission Form by completing the relevant tab(s) for the request: “Primers to Order,” “gDNA,” or “Bacterial Cultures.”
b. The required information for primer design, such as the rs (Reference SNP) number, reference sequence, genome position according to UCSC Genome Browser, etc., if applicable.
c. A copy of the ethics committee approval forms if the submitted samples were obtained from human subjects.
d. A purchase order, if this is the chosen payment method.
Within 24 hours, an automated email from Nanuq is sent:
e. When a new user creates an account, a username and temporary password are assigned.
f. When a new project is opened.
The sequencing and analysis results, if applicable, will thus be accessible directly online in Nanuq.
Sample Submission
Extraction, library preparation, sequencing, genotyping services
Once the service request is completed, submitted and signed, fill the sample submission form.
1. In Nanuq, locate the saved request form in “Requests” and open it.
2. Click the “Sample Submission” tab, then “Add New Samples.”
3. Follow the on-screen instructions.
4. Verify that the submission status is “Submitted” in the “Sample Submission” tab of the Service Request.
Follow the same steps to add new samples to the request or to send replacement samples.
A draft sample submission cannot be approved by the Client Management Office and will not be processed.
For any questions, contact the Client Management Office.
Sanger sequencing service
See the tutorial How to submit samples