{"id":34463,"date":"2026-07-16T21:27:08","date_gmt":"2026-07-17T01:27:08","guid":{"rendered":"https:\/\/genomequebec.com\/?post_type=service&#038;p=34463"},"modified":"2026-07-16T21:41:58","modified_gmt":"2026-07-17T01:41:58","slug":"methylation-arrays-illumina-infinium-methylationepic-technology","status":"publish","type":"service","link":"https:\/\/genomequebec.com\/en\/technological-services\/methylation-arrays-illumina-infinium-methylationepic-technology\/","title":{"rendered":"Methylation Arrays \u2013 Illumina Infinium MethylationEPIC Technology"},"content":{"rendered":"\n<h3 class=\"wp-block-heading has-text-align-center\" id=\"top-of-page\"><em><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-primary-color\">User Guide<\/mark><\/strong><\/em><\/h3>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>The instructions in this guide must be followed carefully to achieve the best possible results and avoid delays in processing your request.<strong>\u202f<\/strong>\u202f&nbsp;<\/p>\n\n\n\n<p>For robust data analysis, it is recommended to process at least 24 samples to ensure proper normalization and correction of batch effects.<\/p>\n\n\n\n<p>For additional information, please contact the <a href=\"https:\/\/genomequebec.com\/en\/contact-us\/\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a>.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" id=\"d\u00e9but-de-page\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"#Starting-Material\" data-type=\"internal\" data-id=\"#Starting-Material\">Starting Material<\/a><\/li>\n\n\n\n<li><a href=\"#Requirements---Quality-and-Quantity\" data-type=\"internal\" data-id=\"#Requirements---Quality-and-Quantity\"><\/a><a href=\"#Requirements---Quality-and-Quantity\" data-type=\"internal\" data-id=\"#Requirements---Quality-and-Quantity\">Requirements \u2013 Quality and Quantity<\/a>\n<ul class=\"wp-block-list\">\n<li><a href=\"#Verification-de-la-concentration\" data-type=\"internal\" data-id=\"#Verification-de-la-concentration\"><\/a><a href=\"#Concentration-Verification\" data-type=\"internal\" data-id=\"#Concentration-Verification\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-black-color\">Concentration Verification<\/mark><\/a><\/li>\n\n\n\n<li><a href=\"#Quality-Verification\" data-type=\"internal\" data-id=\"#Quality-Verification\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-black-color\">Quality Verification<\/mark><\/a><\/li>\n\n\n\n<li class=\"has-black-color has-text-color has-link-color wp-elements-538e347381faefde85116debb8493b68\"><a href=\"#Purety-Verification\" data-type=\"internal\" data-id=\"#Purety-Verification\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-black-color\">Purity Verification<\/mark><\/a><\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><a href=\"#Identification,-Layout-and-Required-Containers\" data-type=\"internal\" data-id=\"#Identification,-Layout-and-Required-Containers\">Identification, Layout and Required Containers<\/a>\n<ul class=\"wp-block-list\">\n<li><a href=\"#Identification\" data-type=\"internal\" data-id=\"#Identification\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-black-color\">Identification<\/mark><\/a><\/li>\n\n\n\n<li><a href=\"#Layout\" data-type=\"internal\" data-id=\"#Layout\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-black-color\">Layout<\/mark><\/a><\/li>\n\n\n\n<li><a href=\"#Required-Containers\" data-type=\"internal\" data-id=\"#Required-Containers\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-black-color\">Required Containers<\/mark><\/a><\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><a href=\"#Service-Request-Form-and-Sample-Submission\" data-type=\"internal\" data-id=\"#Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/a><\/li>\n\n\n\n<li><a href=\"#Sample-Shipment-Preparation\" data-type=\"internal\" data-id=\"#Sample-Shipment-Preparation\">Sample Shipment Preparation<\/a><\/li>\n\n\n\n<li><a href=\"#Transmission-of-results\" data-type=\"internal\" data-id=\"#Transmission-of-results\">Transmission of results<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-f1ea74b888da7196577b0d8ad8e41b53\" id=\"Starting-Material\">Starting Material<\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>Sample Type<\/th><th>Specificity<\/th><\/tr><\/thead><tbody><tr><td>Genomic DNA (gDNA)<\/td><td>No specific treatment required<\/td><\/tr><tr><td>DNA extracted from FFPE tissue blocks<\/td><td>A <a href=\"https:\/\/support.illumina.com\/content\/dam\/illumina-support\/documents\/documentation\/chemistry_documentation\/infinium_assays\/infinium-hd-ffpe\/Inf_FFPE_Sample_Restore_Booklet_15014614_C.pdf\" target=\"_blank\" rel=\"noopener\">restoration<\/a> step will be performed in the laboratory prior to initiating the standard protocol (Illumina Infinium FFPE Sample Restore procedure).<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-bee3914ac10d732d970b4d8b4d00a42f\" id=\"Requirements---Quality-and-Quantity\">Requirements &#8211; Quality and Quantity<\/h4>\n\n\n\n<p>Samples must be eluted in TE 1X buffer (10\u202fmM Tris pH\u202f8.0 \/ 1\u202fmM EDTA) or in nuclease\u2011free ultrapure water.<\/p>\n\n\n\n<p>Note that a fluorometric quantitative analysis is performed upon sample reception to validate DNA concentration.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th class=\"has-text-align-left\" data-align=\"left\">Minimum Volume<\/th><th class=\"has-text-align-left\" data-align=\"left\">Minimum Concentration<\/th><th class=\"has-text-align-left\" data-align=\"left\">Maximum Concentration<\/th><\/tr><\/thead><tbody><tr><td class=\"has-text-align-left\" data-align=\"left\">20&nbsp;uL&nbsp;<\/td><td class=\"has-text-align-left\" data-align=\"left\">35&nbsp;ng\/&nbsp;\u00b5L&nbsp;<\/td><td class=\"has-text-align-left\" data-align=\"left\">200&nbsp;ng\/\u00b5L&nbsp;<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-default\" style=\"margin-top:24px;margin-bottom:24px\"\/>\n\n\n\n<h6 class=\"wp-block-heading has-text-color has-link-color wp-elements-383970138d770b94131ba1cc494c6676\" id=\"Concentration-Verification\">Concentration Verification<\/h6>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Recommended method: <\/strong>Qubit or equivalent fluorometric method.<\/li>\n<\/ul>\n\n\n\n<p><strong>Note:&nbsp; <\/strong>Spectrophotometric methods such as Nanodrop are not recommended for measuring DNA concentration because they tend to overestimate the DNA concentration<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-default\" style=\"margin-top:24px;margin-bottom:24px\"\/>\n\n\n\n<h6 class=\"wp-block-heading has-blue-800-color has-text-color has-link-color wp-elements-bdaf91fbdd822e9143455ca49ba7305f\" id=\"Quality-Verification\">Quality Verification<\/h6>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th class=\"has-text-align-left\" data-align=\"left\">Sample Type<\/th><th class=\"has-text-align-left\" data-align=\"left\">Integrity<\/th><th class=\"has-text-align-left\" data-align=\"left\">Recommended Method<\/th><\/tr><\/thead><tbody><tr><td class=\"has-text-align-left\" data-align=\"left\">Genomic DNA (gDNA)<\/td><td class=\"has-text-align-left\" data-align=\"left\">Fragments&nbsp;&gt;2&nbsp;kb&nbsp;<\/td><td class=\"has-text-align-left\" data-align=\"left\">Electrophoresis<\/td><\/tr><tr><td class=\"has-text-align-left\" data-align=\"left\">DNA extracted from FFPE tissue blocks<\/td><td class=\"has-text-align-left\" data-align=\"left\">N\/A&nbsp;<\/td><td class=\"has-text-align-left\" data-align=\"left\">N\/A&nbsp;<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-default\" style=\"margin-top:24px;margin-bottom:24px\"\/>\n\n\n\n<h6 class=\"wp-block-heading has-text-color has-link-color wp-elements-5b4944fde6d3cdc5319d984d82c14527\" id=\"Purety-Verification\">Purety Verification<\/h6>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Recommended method: <\/strong>Nanodrop or equivalent spectrophotometric method<strong>.<\/strong><\/li>\n\n\n\n<li>Target optical density ratios: &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;\n<ul class=\"wp-block-list\">\n<li>260\/280 between 1,8 et 2,0<\/li>\n\n\n\n<li>260\/230 between 2,0 et 2,2.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-ade3a44bda7032be8f1d2a11447f5590\" id=\"Identification,-Layout-and-Required-Containers\">Identification, Layout and Required Containers<\/h4>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-default\" style=\"margin-top:24px;margin-bottom:24px\"\/>\n\n\n\n<h6 class=\"wp-block-heading has-blue-800-color has-text-color has-link-color wp-elements-87b6b05c712038ac373c46435fee3e65\" id=\"Identification\">Identification<\/h6>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>The name written on the plate must be unique, legible, concise, and strictly identical to the name provided in the sample submission form.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-default\" style=\"margin-top:24px;margin-bottom:24px\"\/>\n\n\n\n<h6 class=\"wp-block-heading has-blue-800-color has-text-color has-link-color wp-elements-a776037f646047bd31331d231803706e\" id=\"Layout\">Layout<\/h6>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Samples must be randomized by row prior to submission.<\/p>\n\n\n\n<p>The figure below illustrates how samples can be arranged for projects involving 24, 48, or 72 samples with 2, 3, or 4 experimental groups:<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"343\" height=\"392\" src=\"https:\/\/genomequebec.com\/wp-content\/uploads\/2026\/07\/Image1.png\" alt=\"\" class=\"wp-image-34462\" srcset=\"https:\/\/genomequebec.com\/wp-content\/uploads\/2026\/07\/Image1.png 343w, https:\/\/genomequebec.com\/wp-content\/uploads\/2026\/07\/Image1-263x300.png 263w\" sizes=\"(max-width: 343px) 100vw, 343px\" \/><\/figure>\n\n\n\n<p>FFPE-derived samples must be handled independently: they must not be included on the same plate as standard genomic DNA and must be randomized separately.<\/p>\n\n\n\n<p>For plates containing human samples, one well must be reserved for the internal positive control:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Full plate: leave well H12 empty<\/li>\n\n\n\n<li>Partially filled plate: leave the first well following the last sample empty, while respecting the required sample multiple for the array.<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-default\" style=\"margin-top:24px;margin-bottom:24px\"\/>\n\n\n\n<h6 class=\"wp-block-heading has-blue-800-color has-text-color has-link-color wp-elements-1b528e243713777dbeeedd910a0825b0\" id=\"Required-Containers\">Required Containers<\/h6>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th class=\"has-text-align-left\" data-align=\"left\">Recommended 96\u2011well PCR Plates<\/th><th class=\"has-text-align-left\" data-align=\"left\">Unaccepted 96\u2011well Plates<\/th><\/tr><\/thead><tbody><tr><td class=\"has-text-align-left\" data-align=\"left\">Full\u2011skirt PCR plates:<br>&#8211; BioRad Hard-Shell 96-Well PCR Plates, skirted, Cat# HSP9601\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<br>&#8211; BioRad Microseal PCR plates 96-well clear, Cat# MSP9601<br>&#8211; Eppendorf twin.tec, Cat# 951020401<br>&#8211; Corning Thermowell GOLD, Cat# 3752\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<br>&#8211; Axygen 96-well PCR Microplate, Cat# PCR96FSC<br><br>Half\u2011skirt PCR plates:<br>&#8211; Life Technologie Thermo-Fast 96 PCR detection plate with flat deck, Cat# AB1400L<br>&#8211; FroggaBio 96-well plate, standard semi-skirted, clear, Cat#SS-96S<\/td><td class=\"has-text-align-left\" data-align=\"left\">&#8211; 96\u2011well cell culture plates<br>&#8211; Opaque PCR plates<br>&#8211; No\u2011skirt PCR plates<\/td><\/tr><tr><td class=\"has-text-align-left\" data-align=\"left\">Recommended adhesive films:<br>&#8211; Adhesive PCR Films; Thermo Fisher Scientific, Cat#AB-0558<br>&#8211; MicroSeal \u2019F\u2019 Foil; Bio-Rad, Cat# MSF-1001<\/td><td class=\"has-text-align-left\" data-align=\"left\"><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-1afbfc3d09342a442ee7af6d6f5edfd7\" id=\"Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/h4>\n\n\n\n<p>Click on&nbsp;<a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" target=\"_blank\" rel=\"noreferrer noopener\">request a service and submit samples<\/a>&nbsp;to view the&nbsp;instructions.&nbsp;<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-096fea86e9448c1a40045488fbf1d398\" id=\"Sample-Shipment-Preparation\">Sample Shipment Preparation<\/h4>\n\n\n\n<p>Click here for&nbsp;instructions&nbsp;on&nbsp;<a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" target=\"_blank\" rel=\"noreferrer noopener\">preparing samples for shipment<\/a>.&nbsp;<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-c198d007b114cae3c4e0c5cfccb510db\" id=\"Transmission-of-results\">Transmission of Results<\/h4>\n\n\n\n<p>An automated message from <a href=\"https:\/\/ces.genomequebec.com\/nanuqAdministration\/index.jsp\" target=\"_blank\" rel=\"noreferrer noopener\">Nanuq<\/a> is sent to the project contacts upon completion of the project.<\/p>\n\n\n\n<p>Analysis files are directly accessible through the Nanuq web application, under the Genotyping Reports section, as well as within the Documents, Analysis Reports, and Downloadable Data tabs.<\/p>\n\n\n\n<p>All samples are retained for three (3) months following the project completion date.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<p><strong>Version 1.0_2026-07-16<\/strong><\/p>\n","protected":false},"featured_media":0,"parent":0,"menu_order":0,"template":"","class_list":["post-34463","service","type-service","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/34463","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/types\/service"}],"wp:attachment":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/media?parent=34463"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}