{"id":34227,"date":"2026-05-23T10:35:37","date_gmt":"2026-05-23T14:35:37","guid":{"rendered":"https:\/\/genomequebec.com\/?post_type=service&#038;p=34227"},"modified":"2026-05-23T11:09:03","modified_gmt":"2026-05-23T15:09:03","slug":"amplicon-library-preparation","status":"publish","type":"service","link":"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/user-guides\/amplicon-library-preparation\/","title":{"rendered":"Amplicon Library Preparation"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-text-align-center\" id=\"top-of-page\"><strong>Illumina<\/strong> <strong>Technology<\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><em>User Guide<\/em><\/h3>\n\n\n\n<p>The instructions in this guide must be followed carefully in order to obtain the best possible results and to avoid any delays in processing your request. Any samples that do not comply with these recommendations may be refused without compensation.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"#Starting-Material\" data-type=\"internal\" data-id=\"#Starting-Material\">Starting Material<\/a><\/li>\n\n\n\n<li><a href=\"#Requirements---Quality-and-Quantity\" data-type=\"internal\" data-id=\"#Requirements---Quality-and-Quantity\">Requirements \u2013 Quantity<\/a><\/li>\n\n\n\n<li><a href=\"#Required-containers-and-sample-identification\" data-type=\"internal\" data-id=\"#Required-containers-and-sample-identification\">Required Containers and Sample Identification<\/a><\/li>\n\n\n\n<li><a href=\"#Service-Request-Form-and-Sample-Submission\" data-type=\"internal\" data-id=\"#Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/a><\/li>\n\n\n\n<li><a href=\"#Sample-Shipment-Preparation\" data-type=\"internal\" data-id=\"#Sample-Shipment-Preparation\">Sample Shipment Preparation<\/a><\/li>\n\n\n\n<li><a href=\"#Transmission-of-results\" data-type=\"internal\" data-id=\"#Transmission-of-results\">Transmission of results<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-f1ea74b888da7196577b0d8ad8e41b53\" id=\"Starting-Material\">Starting Material<\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th><strong>Amplicon Library Preparation<\/strong><\/th><th><strong>Amplicon Indexing<\/strong><\/th><\/tr><\/thead><tbody><tr><td>Genomic DNA (gDNA)<\/td><td>Unpurified PCR product<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-280dbbf76c21d9950ecb85a015567cfe\" id=\"Requirements---Quality-and-Quantity\">Requirements &#8211; Quantity<\/h4>\n\n\n\n<p>Samples must be eluted in 10 mM Tris-HCl buffer, pH 7.5\u20138.5 (without EDTA) or ultrapure nuclease-free water.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th><strong>Sample Type<\/strong><\/th><th><strong>Volume<\/strong><\/th><th><strong>Concentration<\/strong><\/th><th><strong>Recommended method for verifying concentration<\/strong><\/th><\/tr><\/thead><tbody><tr><td>gDNA<\/td><td>10 \u00b5L of gDNA per fragment to be analyzed<\/td><td>1 ng\/\u00b5L<\/td><td>Qubit or equivalent fluorometric method.<br><strong>Note<\/strong>: Spectrophotometric methods such as Nanodrop are much less accurate for measuring DNA concentration. &nbsp;<\/td><\/tr><tr><td>Unpurified PCR product *<\/td><td>20 \u00b5L **<\/td><td>1 ng\/\u00b5L<\/td><td>Qubit or equivalent fluorometric method. <br><strong>Note<\/strong>: Spectrophotometric methods such as Nanodrop are much less accurate for measuring DNA concentration. &nbsp;<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><strong>* Size and integrity must have been verified by gel electrophoresis.<br>** If the minimum sample volume is not met, a diluent will be added to reach the required volume without prior notice<\/strong>.<\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-dbba658f65feaf71a1d85091c8e26301\" id=\"Required-containers-and-sample-identification\">Required Containers and Sample Identification<\/h4>\n\n\n\n<p>Samples submitted in plates that do not meet the requirements will be transferred to an accepted plate format to allow processing. Additional fees will apply.<\/p>\n\n\n\n<p>The name written on the plate must be unique, legible, short, and identical to the one indicated on the submission form.<\/p>\n\n\n\n<p>Samples can be arranged by row or by column. Identification must be written on one side of the plate, not on the seal, to avoid any delay in processing the request.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Recommended Adhesive Films<\/strong><\/td><td><strong>Recommended 96-Well PCR Plates<\/strong><\/td><td><strong>96-Well PCR Plates Not Accepted<\/strong> &nbsp;<\/td><\/tr><tr><td>&#8211; Clear Adhesive PCR Film; Thermo Fisher Scientific, Cat# AB-0558<br>&#8211; Aluminum Foil for PCR and Cold Storage; VWR, Cat# 60941-074<br>&#8211; Tube Cap Strip of 8; Thermo Fisher Scientific, Cat#AB-0265 &nbsp;<\/td><td>Clear, fully skirted PCR plate Examples: <br>&#8211; Eppendorf twin.tec, Cat# 951020401 <br>&#8211; Corning Thermowell GOLD, Cat# 3752 <br>&#8211; Axygen 96-well PCR Microplate, Cat# PCR96FSC<br>&#8211; BioRad Hard-Shell 96-Well PCR Plates, skirted, Cat# HSP9601<br>&#8211; IST 96 well 0.2 mL Skirted PCR plate \u2013 Grey ultra-rigid frame, Cat# IST-601-096GCT<\/td><td>&#8211; Cell culture plate<br>&#8211;&nbsp;No-skirt PCR plates<br>&#8211; Half-skirt PCR plates<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-1afbfc3d09342a442ee7af6d6f5edfd7\" id=\"Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/h4>\n\n\n\n<p>Click on <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/service-request-and-sample-submission\/\" target=\"_blank\" rel=\"noreferrer noopener\">service request and sample submission<\/a>&nbsp;to view instructions.<a id=\"_msocom_1\"><\/a><\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-096fea86e9448c1a40045488fbf1d398\" id=\"Sample-Shipment-Preparation\">Sample Shipment Preparation<\/h4>\n\n\n\n<p>Click on <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" target=\"_blank\" rel=\"noreferrer noopener\">sample shipment preparation<\/a> to view instructions.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-c198d007b114cae3c4e0c5cfccb510db\" id=\"Transmission-of-results\">Transmission of Results<\/h4>\n\n\n\n<p>An automated message from Nanuq is sent to the Principal Investigator (PI) and project contacts as soon as the samples are received, and again at each step of the library preparation process.<\/p>\n\n\n\n<p>Another notification from Nanuq will be sent once sequencing data is available.&nbsp;<\/p>\n\n\n\n<p>Sequencing results are directly accessible via the <a href=\"https:\/\/ces.genomequebec.com\/nanuqAdministration\/j_security_check?service=http%3A%2F%2Fces.genomequebec.com%2FnanuqAdministration%2Flogin.do\" target=\"_blank\" rel=\"noreferrer noopener\">Nanuq<\/a> web application.<\/p>\n\n\n\n<p><a id=\"_msocom_1\"><\/a><\/p>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong><strong>Version 2.0_2026-05-15<\/strong><\/strong><\/p>\n","protected":false},"featured_media":0,"parent":23840,"menu_order":0,"template":"","class_list":["post-34227","service","type-service","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/34227","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/types\/service"}],"up":[{"embeddable":true,"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/23840"}],"wp:attachment":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/media?parent=34227"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}