{"id":34190,"date":"2026-05-12T16:24:41","date_gmt":"2026-05-12T20:24:41","guid":{"rendered":"https:\/\/genomequebec.com\/?post_type=service&#038;p=34190"},"modified":"2026-05-13T14:41:37","modified_gmt":"2026-05-13T18:41:37","slug":"proteomics-services-illumina-protein-prep","status":"publish","type":"service","link":"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/user-guides\/proteomics-services-illumina-protein-prep\/","title":{"rendered":"Proteomics Services &#8211; Illumina Protein Prep"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-text-align-center\" id=\"top-of-page\"><strong><strong><em>Illumina Protein Prep <\/em>Technology<\/strong><\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><em>User Guide<\/em><\/h3>\n\n\n\n<p>The instructions in this guide must be followed carefully to achieve the best possible results and avoid delays in processing your request.<\/p>\n\n\n\n<p>Samples that do not comply with the recommendations may be rejected without compensation.<\/p>\n\n\n\n<p>Note that delays in the processing of samples will vary depending on the size of the project. It is recommended to contact the\u00a0<a href=\"https:\/\/genomequebec.com\/en\/contact-us\/\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a>\u00a0for information regarding processing time.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"#General-Information\" data-type=\"internal\" data-id=\"#General-Information\">General Information<\/a>\n<ul class=\"wp-block-list\">\n<li><a href=\"#Importance-of-sample-collection-and-processing-conditions\" data-type=\"internal\" data-id=\"#Importance-of-sample-collection-and-processing-conditions\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-black-color\">Importance of sample collection and processing conditions<\/mark><\/a><\/li>\n\n\n\n<li><a href=\"#Blood-sample-collection-and-preparation\" data-type=\"internal\" data-id=\"#Blood-sample-collection-and-preparation\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-black-color\">Blood sample collection and preparation<\/mark><\/a><\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><a href=\"#Starting-Material\" data-type=\"internal\" data-id=\"#Starting-Material\">Starting Material<\/a><\/li>\n\n\n\n<li><a href=\"#Requirements-Volume\" data-type=\"internal\" data-id=\"#Requirements-Volume\">Requirements \u2013 Volumes<\/a><\/li>\n\n\n\n<li><a href=\"#Required-containers-and-sample-identification\" data-type=\"internal\" data-id=\"#Required-containers-and-sample-identification\">Required Containers and Sample Identification<\/a><\/li>\n\n\n\n<li><a href=\"#Service-Request-Form-and-Sample-Submission\" data-type=\"internal\" data-id=\"#Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/a><\/li>\n\n\n\n<li><a href=\"#Sample-Shipment-Preparation\" data-type=\"internal\" data-id=\"#Sample-Shipment-Preparation\">Sample Shipment Preparation<\/a><\/li>\n\n\n\n<li><a href=\"#Transmission-of-results\" data-type=\"internal\" data-id=\"#Transmission-of-results\">Transmission of results<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-0b7c816007d7a1ffff7d811c47a73b00\" id=\"General-Information\">General Information<\/h4>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-default\" style=\"margin-top:24px;margin-bottom:24px\"\/>\n\n\n\n<h6 class=\"wp-block-heading has-blue-800-color has-text-color has-link-color wp-elements-71098db81bba146ae9e2e99693d4ec00\" id=\"Importance-of-sample-collection-and-processing-conditions\"><strong>Importance of sample collection and processing conditions<\/strong><\/h6>\n\n\n\n<p>Plasma and serum proteins are complex matrices whose composition can be significantly influenced by pre-analytical conditions.<\/p>\n\n\n\n<p>Variations in collection methods and tube types, processing delays, freeze\u2013thaw cycles, or storage containers may alter the protein profiles detected by the Illumina Protein Prep technology, thereby compromising the comparability of results between samples or across projects.<br><br>For more information on the importance of collection and processing conditions and their impact on results, please refer to the following resource&nbsp;: <a href=\"https:\/\/knowledge.illumina.com\/library-preparation\/multiomics-library-prep\/library-preparation-multiomics-library-prep-faq-list\/000009781\" target=\"_blank\" rel=\"noopener\">Input and sample collection for the Illumina Protein Prep (IPP) | Illumina Knowledge<\/a><a id=\"_msocom_1\"><\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-default\" style=\"margin-top:24px;margin-bottom:24px\"\/>\n\n\n\n<h6 class=\"wp-block-heading has-blue-800-color has-text-color has-link-color wp-elements-87ad470aa268d05a4b638ed0800d806d\" id=\"Blood-sample-collection-and-preparation\"><strong>Blood sample collection and preparation<\/strong><\/h6>\n\n\n\n<p>The collection conditions outlined below reflect Illumina-validated recommendations for the Illumina Protein Preparation technology. For any collection performed using other tube types or under different conditions, contact the&nbsp;<a href=\"https:\/\/genomequebec.com\/en\/contact-us\/\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a>.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th class=\"has-text-align-center\" data-align=\"center\"><strong>Parameters<\/strong><\/th><th class=\"has-text-align-center\" data-align=\"center\"><strong>Plasma<\/strong><\/th><th class=\"has-text-align-center\" data-align=\"center\"><strong>Serum<\/strong><\/th><\/tr><\/thead><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Recommended collection tube type<\/mark><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>EDTA <\/strong>tube<\/td><td class=\"has-text-align-center\" data-align=\"center\">Non- anticoagulant tube<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Processing time<\/mark><\/td><td class=\"has-text-align-center\" data-align=\"center\">Process within 30 minutes after collection<\/td><td class=\"has-text-align-center\" data-align=\"center\">Allow to clot for 60\u201390 minutes at R.T. before centrifugation<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Centrifugation<\/mark><\/td><td class=\"has-text-align-center\" data-align=\"center\">2200 \u00d7 g, <strong>15 min<\/strong> at R.T<\/td><td class=\"has-text-align-center\" data-align=\"center\">2200 \u00d7 g, <strong>15 min<\/strong> at R.T.<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Fraction to collect<\/mark><\/td><td class=\"has-text-align-center\" data-align=\"center\">Plasma \u2013 avoid the buffy coat layer<\/td><td class=\"has-text-align-center\" data-align=\"center\">Serum<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Aliquotage<\/mark><\/td><td class=\"has-text-align-center\" data-align=\"center\">Immediately after centrifugation<\/td><td class=\"has-text-align-center\" data-align=\"center\">Within 30 minutes after centrifugation<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Conservation<\/mark><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u201380 \u00b0C<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u201380 \u00b0C<\/strong><\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Freeze\/thaw cycles<\/mark><\/td><td class=\"has-text-align-center\" data-align=\"center\">Minimize (Recommended: maximum of 2 cycles)<\/td><td class=\"has-text-align-center\" data-align=\"center\">Minimize (Recommended: maximum of 2 cycles)<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\">R.T.&nbsp;: room temperature<\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-f1ea74b888da7196577b0d8ad8e41b53\" id=\"Starting-Material\">Starting Material<\/h4>\n\n\n\n<p>The starting material is plasma or serum.<\/p>\n\n\n\n<p>Do not mix sample types (plasma and serum) within the same service request, as they require distinct protocols and cannot be processed simultaneously.<\/p>\n\n\n\n<p>The presence of hemolysis in a sample may affect protein profiles.<\/p>\n\n\n\n<p>It is recommended that samples within the same project undergo a similar number of freeze\u2013thaw cycles and a consistent pre-analytical processing workflow. If in doubt, contact the&nbsp;<a href=\"https:\/\/genomequebec.com\/en\/contact-us\/\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a>.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-c304c8e2ceeac835429f2aaea4ed5720\" id=\"Requirements-Volume\">Requirements &#8211; Volume<\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th><strong>Requirement<\/strong><\/th><th><strong>Minimum<\/strong><\/th><th><strong>Recommand<\/strong>ed<\/th><\/tr><\/thead><tbody><tr><td>Volume<\/td><td>\u226555 \u00b5L per sample<\/td><td>100 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The volume entered in the sample submission form must exactly match the physical volume in the plate.<\/li>\n\n\n\n<li>Replacement samples must meet total requirements.<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-dbba658f65feaf71a1d85091c8e26301\" id=\"Required-containers-and-sample-identification\">Required Containers and Sample Identification<\/h4>\n\n\n\n<p><strong>Recommendation:<\/strong> use low-protein-binding plastic containers (e.g., certified low-binding microtubes or cryovials) to minimize non-specific adsorption and maximize protein recovery.<\/p>\n\n\n\n<p><br><strong>Important<\/strong> <strong>:<\/strong> if your samples are stored in a different type of container, please contact the <a href=\"https:\/\/genomequebec.com\/en\/contact-us\/\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a> prior to any thawing or sample transfer to confirm compatibility.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong><strong>Recommended containers<\/strong><\/strong><\/td><td><strong><strong>Accepted containers<\/strong><\/strong><\/td><\/tr><tr><td>2D Matrix plates <br><br>&#8211; Cat# 3744-WP1D-BR<\/td><td>&#8211; 1.5mL tubes<br>&#8211; 2mL Cryovials<br>&#8211; 96-well PCR plates<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Each 96-well plate must correspond to a single submission form and be labeled exactly as indicated.<\/li>\n\n\n\n<li>Samples must be arranged in the plate according to the layout specified in the sample submission form.<\/li>\n<\/ul>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-1afbfc3d09342a442ee7af6d6f5edfd7\" id=\"Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/h4>\n\n\n\n<p>Click on <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/service-request-and-sample-submission\/\" target=\"_blank\" rel=\"noreferrer noopener\">request a service and submit samples<\/a> to view the instructions.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-096fea86e9448c1a40045488fbf1d398\" id=\"Sample-Shipment-Preparation\">Sample Shipment Preparation<\/h4>\n\n\n\n<p>Click here for instructions on <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" target=\"_blank\" rel=\"noreferrer noopener\">preparing samples for shipment<\/a>.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-c198d007b114cae3c4e0c5cfccb510db\" id=\"Transmission-of-results\">Transmission of Results<\/h4>\n\n\n\n<p>The Dragen Protein Quantification report, as well as a normalized count file in ADAT format, will be provided via <a href=\"https:\/\/ces.genomequebec.com\/nanuqAdministration\/nanuq-administration\/welcomeHome.do\">Nanuq<\/a>. Additional analyses such as differential expression or multi-omics comparisons are available upon request.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<p><strong>Version 1.0_2026-04-21<\/strong><\/p>\n","protected":false},"featured_media":0,"parent":23840,"menu_order":0,"template":"","class_list":["post-34190","service","type-service","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/34190","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/types\/service"}],"up":[{"embeddable":true,"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/23840"}],"wp:attachment":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/media?parent=34190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}