{"id":34186,"date":"2026-05-12T12:39:10","date_gmt":"2026-05-12T16:39:10","guid":{"rendered":"https:\/\/genomequebec.com\/?post_type=service&#038;p=34186"},"modified":"2026-05-12T17:35:26","modified_gmt":"2026-05-12T21:35:26","slug":"library-preparation-from-rna","status":"publish","type":"service","link":"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/user-guides\/library-preparation-from-rna\/","title":{"rendered":"Library Preparation from RNA"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-text-align-center\" id=\"top-of-page\"><strong>Illumina Technology<\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><em>User Guide<\/em><\/h3>\n\n\n\n<p>The instructions in this guide must be followed carefully to achieve the best possible results and avoid delays in processing your request.<\/p>\n\n\n\n<p>Any samples that do not comply with the recommendations may be rejected without compensation.<\/p>\n\n\n\n<p>Note that delays in the processing of libraries will vary depending on the size of the project. It is recommended to contact the <a href=\"https:\/\/genomequebec.com\/en\/contact-us\/\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a> for information&nbsp;regarding&nbsp;processing time.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h5 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-063f7f5ad7ee22649cb4eb3a92c4fc4c\"><em>Quick search below<\/em><\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"#Starting-Material\" data-type=\"internal\" data-id=\"#Starting-Material\">Starting Material<\/a><\/li>\n\n\n\n<li><a href=\"#Requirements---Quality-and-Quantity\" data-type=\"internal\" data-id=\"#Requirements---Quality-and-Quantity\">Requirements \u2013 Quality and Quantity<\/a><\/li>\n\n\n\n<li><a href=\"#Required-containers-and-sample-identification\" data-type=\"internal\" data-id=\"#Required-containers-and-sample-identification\">Required Containers and Sample Identification<\/a><\/li>\n\n\n\n<li><a href=\"#Service-Request-Form-and-Sample-Submission\" data-type=\"internal\" data-id=\"#Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/a><\/li>\n\n\n\n<li><a href=\"#Sample-Shipment-Preparation\" data-type=\"internal\" data-id=\"#Sample-Shipment-Preparation\">Sample Shipment Preparation<\/a><\/li>\n\n\n\n<li><a href=\"#Transmission-of-results\" data-type=\"internal\" data-id=\"#Transmission-of-results\">Transmission of results<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-f1ea74b888da7196577b0d8ad8e41b53\" id=\"Starting-Material\">Starting Material<\/h4>\n\n\n\n<p>Total RNA is used as the starting material for all protocols.&nbsp;<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h6 class=\"wp-block-heading\"><strong>Practical advice and good laboratory practices<\/strong><\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Samples should be resuspended in commercial RNase\u2011free water or in the elution buffer provided in commercial kits.&nbsp;&nbsp;<\/li>\n\n\n\n<li>Samples should be treated with DNase to eliminate the possibility of DNA contamination.&nbsp;<\/li>\n\n\n\n<li>If Trizol is used to extract RNA samples, a final cleanup should be performed (e.g., using a column\u2011based cleanup kit) before submitting the samples.&nbsp;&nbsp;<\/li>\n\n\n\n<li>DEPC\u2011treated water should be avoided. Residual DEPC degradation products can inhibit subsequent reactions.&nbsp;&nbsp;<\/li>\n\n\n\n<li>RNA should not be resuspended in solutions containing detergents (e.g., SDS), as these can interfere with enzymatic reactions.&nbsp;<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-bee3914ac10d732d970b4d8b4d00a42f\" id=\"Requirements---Quality-and-Quantity\">Requirements &#8211; Quality and Quantity<\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th><strong>Library preparation type<\/strong>&nbsp;<\/th><th><strong>Quantity&nbsp;<\/strong>&nbsp;<\/th><th><strong>Concentration<\/strong>&nbsp;<\/th><th><strong>Volume<\/strong>&nbsp;<\/th><th><strong>Quality*<\/strong><\/th><\/tr><\/thead><tbody><tr><td>Poly(A)&nbsp;Selection&nbsp;<\/td><td>\u2265300&nbsp;ng&nbsp;<\/td><td>\u226510&nbsp;ng\/\u00b5L&nbsp;<\/td><td>\u226530&nbsp;\u00b5L&nbsp;<\/td><td>RIN \u22656.5&nbsp;Ratio&nbsp;260:280 1.8-2.1&nbsp;Ratio&nbsp;260:230 1.8-2.2&nbsp;<\/td><\/tr><tr><td>Poly(A) Selection + globin&nbsp;Depletion&nbsp;<\/td><td>\u2265300&nbsp;ng&nbsp;<\/td><td>\u226510&nbsp;ng\/\u00b5L&nbsp;<\/td><td>\u226530&nbsp;\u00b5L&nbsp;<\/td><td>RIN \u22656.5&nbsp;Ratio&nbsp;260:280 1.8-2.1&nbsp;Ratio&nbsp;260:230 1.8-2.2&nbsp;<\/td><\/tr><tr><td>Low Input Poly-(A) Selection (non&nbsp;directional, for quantity lower&nbsp;than 25ng)&nbsp;&nbsp;<\/td><td>\u22650.5&nbsp;ng&nbsp;<\/td><td>\u22650.06&nbsp;ng\/\u00b5L&nbsp;<\/td><td>\u226510 \u00b5L&nbsp;<\/td><td>RIN \u22656.5&nbsp;Ratio&nbsp;260:280 1.8-2.1&nbsp;Ratio 260:230 1.8-2.2&nbsp;<\/td><\/tr><tr><td>Bacterial rRNA&nbsp;Depletion&nbsp;and&nbsp;Meta-transcriptomic rRNA Depletion (bacterial rRNA +&nbsp;host)&nbsp;&nbsp;<\/td><td>\u2265200&nbsp;ng&nbsp;<\/td><td>\u226510&nbsp;ng\/\u00b5L&nbsp;<\/td><td>\u226520&nbsp;\u00b5L&nbsp;<\/td><td>RIN \u22656.5&nbsp;Ratio&nbsp;260:280 1.8-2.1&nbsp;Ratio 260:230 1.8-2.2&nbsp;<\/td><\/tr><tr><td>rRNA Depletion from different species (Human, mouse, rat, plant, fish, etc.)&nbsp;<\/td><td>\u2265320&nbsp;ng&nbsp;<\/td><td>\u226532&nbsp;ng\/\u00b5L&nbsp;<\/td><td>\u226510&nbsp;\u00b5L&nbsp;<\/td><td>RIN \u22656.5&nbsp;Ratio&nbsp;260:280 1.8-2.1&nbsp;Ratio 260:230 1.8-2.2&nbsp;<\/td><\/tr><tr><td>Small RNA profiling (&lt;200 nt) and\/or miRNA (21\u201325 nt)&nbsp;<\/td><td>\u2265750&nbsp;ng&nbsp;<\/td><td>\u226563&nbsp;ng\/\u00b5L&nbsp;<\/td><td>\u226512&nbsp;\u00b5L&nbsp;<\/td><td>RIN \u22656.5&nbsp;Ratio&nbsp;260:280 1.8-2.1&nbsp;Ratio 260:230 1.8-2.2&nbsp;<\/td><\/tr><tr><td>Total RNA&nbsp;&nbsp;<\/td><td>\u226550&nbsp;ng&nbsp;<\/td><td>\u226510&nbsp;ng\/\u00b5L&nbsp;<\/td><td>\u22655&nbsp;\u00b5L&nbsp;<\/td><td>RIN \u22656.5&nbsp;Ratio&nbsp;260:280 1.8-2.1&nbsp;Ratio 260:230 1.8-2.2&nbsp;<\/td><\/tr><tr><td>RNA exome&nbsp;<\/td><td>\u226520&nbsp;ng&nbsp;<\/td><td>\u22651.2&nbsp;ng\/\u00b5L&nbsp;<\/td><td>\u226512&nbsp;\u00b5L&nbsp;<\/td><td>RIN \u22656.5&nbsp;Ratio&nbsp;260:280 1.8-2.1&nbsp;Ratio 260:230 1.8-2.2&nbsp;<\/td><\/tr><tr><td>RNA exome (FFPE RNA)&nbsp;<\/td><td>\u226530&nbsp;ng&nbsp;<\/td><td>\u22652.4&nbsp;ng\/\u00b5L&nbsp;<\/td><td>\u226512&nbsp;\u00b5L&nbsp;<\/td><td>DV200 \u226536.5&nbsp;<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\">&nbsp;<br>*&nbsp;RIN = RNA Integrity Number&nbsp;<br>&nbsp;<br>&#8211; The use of out\u2011of\u2011specification samples may limit the detection of low\u2011expressed genes, compromising the sensitivity and representativeness of the analysis.&nbsp;&nbsp;<br>&#8211; The RIN value for rRNA\u2011depletion protocols may be lower than 6.5. Contact a member of the&nbsp;<a href=\"mailto:infoservices@genomequebec.com\" target=\"_blank\" rel=\"noreferrer noopener\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Client Management Office<\/mark><\/a>&nbsp;to discuss this.&nbsp;<br>&#8211; The platform\u2019s ability to generate small RNA libraries may vary depending on sample characteristics and experimental conditions. Contact a member of the&nbsp;<a href=\"mailto:infoservices@genomequebec.com\" target=\"_blank\" rel=\"noreferrer noopener\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Client Management Office<\/mark><\/a>&nbsp;to discuss this.&nbsp;<br><strong>Replacement samples are accepted and must meet the same criteria as the original samples. Partial replacements (\u2018top<\/strong>\u2011<strong>ups\u2019) are not accepted.<\/strong>&nbsp;<\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-dbba658f65feaf71a1d85091c8e26301\" id=\"Required-containers-and-sample-identification\">Required Containers and Sample Identification<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>It is recommended to send samples in low\u2011binding tubes.&nbsp;<\/li>\n\n\n\n<li>The name written on the tube must be unique, legible, short, and identical to the one indicated on the submission form.&nbsp;<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th><strong>Accepted Formats<\/strong>&nbsp;<\/th><th><strong>Unaccepted formats<\/strong>&nbsp;<\/th><\/tr><\/thead><tbody><tr><td>1.5mL&nbsp;Tubes&nbsp;2mL&nbsp;Tubes&nbsp;<\/td><td>Strip Tubes&nbsp;0.2mL&nbsp;Tubes&nbsp;&nbsp;0.5mL&nbsp;Tubes&nbsp;&nbsp;2D&nbsp;Tubes&nbsp;All plate formats&nbsp;<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-1afbfc3d09342a442ee7af6d6f5edfd7\" id=\"Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/h4>\n\n\n\n<p>Click on <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" target=\"_blank\" rel=\"noreferrer noopener\">request a service and submit samples<\/a> to view the instructions.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-096fea86e9448c1a40045488fbf1d398\" id=\"Sample-Shipment-Preparation\">Sample Shipment Preparation<\/h4>\n\n\n\n<p>Click on <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" data-type=\"link\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" target=\"_blank\" rel=\"noreferrer noopener\">samples shipment preparation<\/a> to view the instructions.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-c198d007b114cae3c4e0c5cfccb510db\" id=\"Transmission-of-results\">Transmission of Results<\/h4>\n\n\n\n<p>A message is sent to all contact persons listed in the Nanuq project as soon as the results of the initial sample quality control are available. The same applies when the library quality control results become available<\/p>\n\n\n\n<p>An automated notification from Nanuq will be sent to the principal investigator and project contacts once sequencing data are available.<\/p>\n\n\n\n<p>Sequencing results are directly accessible via the <a href=\"https:\/\/ces.genomequebec.com\/nanuqAdministration\/j_security_check?service=https%3A%2F%2Fces.genomequebec.com%2FnanuqAdministration%2F\" target=\"_blank\" rel=\"noreferrer noopener\">Nanuq<\/a> web application.<\/p>\n\n\n\n<p>For more information on downloading data and available files, please refer to the <a href=\"https:\/\/ces.genomequebec.com\/nanuqAdministration\/faq.go\" target=\"_blank\" rel=\"noreferrer noopener\">Frequently Asked Questions<\/a> page.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong><strong>Version 2.0_2026-04-21<\/strong><\/strong><\/p>\n","protected":false},"featured_media":0,"parent":23840,"menu_order":0,"template":"","class_list":["post-34186","service","type-service","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/34186","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/types\/service"}],"up":[{"embeddable":true,"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/23840"}],"wp:attachment":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/media?parent=34186"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}