{"id":34178,"date":"2026-05-12T11:03:15","date_gmt":"2026-05-12T15:03:15","guid":{"rendered":"https:\/\/genomequebec.com\/?post_type=service&#038;p=34178"},"modified":"2026-05-12T17:34:02","modified_gmt":"2026-05-12T21:34:02","slug":"library-preparation-from-dna","status":"publish","type":"service","link":"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/user-guides\/library-preparation-from-dna\/","title":{"rendered":"Library Preparation from DNA"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-text-align-center\" id=\"top-of-page\"><strong>Illumina Technology<\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><em>User Guide<\/em><\/h3>\n\n\n\n<p>The instructions in this guide must be followed carefully to achieve the best possible results and avoid delays in processing your request.<\/p>\n\n\n\n<p>Any samples that do not comply with the recommendations may be rejected without compensation.<\/p>\n\n\n\n<p>Note that delays in the processing of libraries will vary depending on the size of the project. It is recommended to contact the <a href=\"https:\/\/genomequebec.com\/en\/contact-us\/\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a> for information&nbsp;regarding&nbsp;processing time.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h5 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-063f7f5ad7ee22649cb4eb3a92c4fc4c\"><em>Quick search below<\/em><\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"#Starting-Material\" data-type=\"internal\" data-id=\"#Starting-Material\">Starting Material<\/a><\/li>\n\n\n\n<li><a href=\"#Requirements---Quality-and-Quantity\" data-type=\"internal\" data-id=\"#Requirements---Quality-and-Quantity\">Requirements \u2013 Quality and Quantity<\/a><\/li>\n\n\n\n<li><a href=\"#Required-containers-and-sample-identification\" data-type=\"internal\" data-id=\"#Required-containers-and-sample-identification\">Required Containers and Sample Identification<\/a><\/li>\n\n\n\n<li><a href=\"#Service-Request-Form-and-Sample-Submission\" data-type=\"internal\" data-id=\"#Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/a><\/li>\n\n\n\n<li><a href=\"#Sample-Shipment-Preparation\" data-type=\"internal\" data-id=\"#Sample-Shipment-Preparation\">Sample Shipment Preparation<\/a><\/li>\n\n\n\n<li><a href=\"#Transmission-of-results\" data-type=\"internal\" data-id=\"#Transmission-of-results\">Transmission of results<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-f1ea74b888da7196577b0d8ad8e41b53\" id=\"Starting-Material\">Starting Material<\/h4>\n\n\n\n<p>Genomic DNA is usually the material used for all protocols except the circulating DNA protocol. If the starting material is different, you must contact a member of the <a href=\"mailto:infoservices@genomequebec.com\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a> to discuss feasibility.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h6 class=\"wp-block-heading\"><strong>Practical advice and good laboratory practices<\/strong><\/h6>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Samples should exhibit the highest possible quality and purity. Quality is not assessed at the CES; only quantification is performed<\/li>\n\n\n\n<li>DNA concentration should be measured using fluorometric methods (e.g., Qubit, PicoGreen) rather than spectrophotometric methods (e.g., Nanodrop), which tend to overestimate sample concentration and may result in an inadequate amount of starting material.<\/li>\n\n\n\n<li>DNA should be extracted using commercial kits rather than homemade solutions.<\/li>\n\n\n\n<li>If Trizol is used to extract DNA samples, a final cleanup should be performed (e.g., using a column\u2011based cleanup kit) before submitting the samples.<\/li>\n\n\n\n<li>Samples should be treated with RNase to eliminate the possibility of RNA contamination.<\/li>\n\n\n\n<li>DNA should be resuspended in 10 mM Tris\u2011HCl pH 8.0; 0.1 mM EDTA, in commercial RNase\/DNase\u2011free water, or in the elution buffer provided in commercial kits.<\/li>\n\n\n\n<li>Samples should not contain insoluble material, RNA, chelating agents (e.g., EDTA), divalent cations, phenol, detergents, or contaminants originating from the source material (organism or tissue).<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-bee3914ac10d732d970b4d8b4d00a42f\" id=\"Requirements---Quality-and-Quantity\">Requirements &#8211; Quality and Quantity<\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th><strong>Library preparation type<\/strong><\/th><th><strong>Quantity<\/strong><\/th><th><strong>Concentration<\/strong><\/th><th><strong>Volume<\/strong><\/th><th><strong>Quality<\/strong><\/th><\/tr><\/thead><tbody><tr><td>Shotgun with PCR<\/td><td>\u2265150ng<\/td><td>\u22655 ng\/\u00b5L<\/td><td>\u226530 \u00b5L<\/td><td>Ratio 260:280 1.8-2.0 Ratio 260:230 \u22652.0<\/td><\/tr><tr><td>PCR Free Shotgun<\/td><td>\u2265600ng<\/td><td>\u226520 ng\/\u00b5L<\/td><td>\u226530 \u00b5L<\/td><td>Ratio 260:280 1.8-2.0 Ratio 260:230 \u22652.0<\/td><\/tr><tr><td>Methyl-seq<\/td><td>\u2265150 ng<\/td><td>\u22655 ng\/\u00b5L<\/td><td>\u226530 \u00b5L<\/td><td>Ratio 260:280 1.8-2.0 Ratio 260:230 \u22652.0<\/td><\/tr><tr><td>Whole Exome (Agilent SureSelect Probes)<\/td><td>\u2265150 ng<\/td><td>\u22655 ng\/\u00b5L<\/td><td>\u226530 \u00b5L<\/td><td>Ratio 260:280 1.8-2.0 Ratio 260:230 \u22652.0<\/td><\/tr><tr><td>Whole Exome (Roche KAPA HyperExome Probes)<\/td><td>\u2265150 ng<\/td><td>\u22655 ng\/\u00b5L<\/td><td>\u226530 \u00b5L<\/td><td>Ratio 260:280 1.8-2.0 Ratio 260:230 \u22652.0<\/td><\/tr><tr><td>cfDNA libraries<\/td><td>\u226530 ng<\/td><td>\u22651 ng\/\u00b5L<\/td><td>\u226530 \u00b5L<\/td><td>Ratio 260:280 1.8-2.0 Ratio 260:230 \u22652.0<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><strong>Replacement samples are accepted and must meet the same criteria as the original samples. Partial replacements (\u2018top\u2011ups\u2019) are not accepted.<\/strong><\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-dbba658f65feaf71a1d85091c8e26301\" id=\"Required-containers-and-sample-identification\">Required Containers and Sample Identification<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Samples must be sent in 96\u2011well plates, regardless of the number of samples<\/li>\n\n\n\n<li>Each plate must contain only samples belonging to a single Nanuq project.&nbsp;<\/li>\n\n\n\n<li>Each plate must be clearly identified, and the samples must be placed exactly as indicated in the sample submission form.<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th><strong><strong>Accepted Formats<\/strong><\/strong><\/th><th><strong><strong>Unaccepted Formats<\/strong><\/strong><\/th><\/tr><\/thead><tbody><tr><td>Recommanded full skirt plates&nbsp;:<strong><br><\/strong><br>&#8211; BioRad&nbsp;Hard-Shell 96-Well PCR Plates, skirted, Cat# HSP9601&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;<br>&#8211; Eppendorf&nbsp;twin.tec, Cat# 951020401&nbsp;<br>&#8211; Corning Thermowell GOLD, Cat# 3752&nbsp;<\/td><td>&#8211; Half-skirt plates<br>&#8211; Non skirted plates<br>&#8211; Cell culture plates<\/td><\/tr><tr><td>Recommanded adhesive films<br>&nbsp;<br>&#8211; Life Technologies MicroAmp\u00ae Clear Adhesive Film, Cat# 4306311<br>&#8211; Thermo Scientific\u2122 Adhesive PCR Plate Seals, Cat# AB0558<br>&#8211; VWR Aluminum Foils for PCR and Cold Storage, Cat# 60941-074<\/td><td>&nbsp;<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-1afbfc3d09342a442ee7af6d6f5edfd7\" id=\"Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/h4>\n\n\n\n<p>Click on <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" target=\"_blank\" rel=\"noreferrer noopener\">request a service and submit samples<\/a> to view the instructions.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-096fea86e9448c1a40045488fbf1d398\" id=\"Sample-Shipment-Preparation\">Sample Shipment Preparation<\/h4>\n\n\n\n<p>Click on <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" rel=\"noreferrer noopener\">Samples shipment preparation<\/a> to view the instructions.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-c198d007b114cae3c4e0c5cfccb510db\" id=\"Transmission-of-results\">Transmission of Results<\/h4>\n\n\n\n<p>A message is sent to all contact persons listed in the Nanuq project as soon as the results of the initial sample quality control are available. The same applies when the library quality control results become available<\/p>\n\n\n\n<p>An automated notification from Nanuq will be sent to the principal investigator and project contacts once sequencing data are available.<\/p>\n\n\n\n<p>Sequencing results are directly accessible via the <a href=\"https:\/\/ces.genomequebec.com\/nanuqAdministration\/j_security_check?service=https%3A%2F%2Fces.genomequebec.com%2FnanuqAdministration%2F\">Nanuq<\/a> web application.<\/p>\n\n\n\n<p>For more information on downloading data and available files, please refer to the <a href=\"https:\/\/ces.genomequebec.com\/nanuqAdministration\/faq.go\">Frequently Asked Questions<\/a> page.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong><strong>Version 2.0_2026-04-21<\/strong><\/strong><\/p>\n","protected":false},"featured_media":0,"parent":23840,"menu_order":0,"template":"","class_list":["post-34178","service","type-service","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/34178","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/types\/service"}],"up":[{"embeddable":true,"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/23840"}],"wp:attachment":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/media?parent=34178"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}