{"id":32036,"date":"2025-07-15T14:37:54","date_gmt":"2025-07-15T18:37:54","guid":{"rendered":"https:\/\/genomequebec.com\/?post_type=service&p=32036"},"modified":"2025-11-27T10:48:15","modified_gmt":"2025-11-27T15:48:15","slug":"long-pcr-product-and-whole-plasmid-sequencing-service-using-oxford-nanopore-technology","status":"publish","type":"service","link":"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/sequencing\/long-pcr-product-and-whole-plasmid-sequencing-service-using-oxford-nanopore-technology\/","title":{"rendered":"Long PCR product and whole plasmid sequencing service using Oxford Nanopore technology"},"content":{"rendered":"\n
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The CES offers a standard whole plasmid sequencing service (less than 25 kb) and long PCR product sequencing (more than 1500 bp and less than 25 kb) using Oxford Nanopore technology.<\/p>\n\n\n\n

To find out when to choose the PlasmiQ service versus the Sanger sequencing service, see the following table:<\/p>\n\n\n\n

Criteria<\/strong><\/td>\"\"<\/td>Sanger sequencing<\/strong><\/td><\/tr>
Price per reaction<\/strong><\/td>$20<\/td>~$4 to $8 (several options available)<\/td><\/tr>
Purification<\/strong><\/td>Additional fees<\/td>Included<\/td><\/tr>
Applications<\/strong><\/td>Sequence an insert or a complete plasmid, even without a known sequence. Effectively verify the integrity of plasmids that have been in use for a long time.<\/td>Fast and economical reading of short fragments already known. Ideal for verifying known mutations, quickly confirming cloning, sequencing an insert <1500 bp.<\/td><\/tr>
Use of primers<\/strong><\/td>No specific primers required<\/td>Specific primers required<\/td><\/tr>
Turnaround time<\/strong><\/td>3 to 7 days (3 days for samples received on Tuesday before 4 p.m.) \u2013 Starting service \u2013 Turnaround time subject to change<\/td>~24 hours to 3 days<\/td><\/tr>
Sequencing scope<\/strong><\/td>Sequencing of entire molecules with continuous long reads, allowing for complete structural analysis.<\/td>Targeted sequencing of a given region with an average accuracy of 99.99%.<\/td><\/tr>
Read length<\/strong><\/td>1500 pb to 25 kb<\/td>100 pb to 800 pb<\/td><\/tr>
Sequencing bias<\/strong><\/td>No bias related to primers\u2014the entire molecule is analyzed.<\/td>Risk of bias if the primer does not hybridize perfectly, which may mask certain mutations.<\/td><\/tr>
SNV detection<\/strong><\/td>Detects low-frequency SNVs.<\/td>Detects high-frequency SNVs (>25%)<\/td><\/tr>
Structural variations<\/strong><\/td>Detects the sites and sequence of rearrangements and duplications.<\/td>Identifies the insertion sites of rearrangements and duplications.<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

For more details, see the user guide<\/a>.<\/p>\n\n\n\n

Oxford Nanopore technology is also used for other applications:<\/p>\n\n\n\n