{"id":23862,"date":"2023-03-27T15:17:39","date_gmt":"2023-03-27T19:17:39","guid":{"rendered":"https:\/\/genomequebec.com\/?post_type=service&#038;p=23862"},"modified":"2023-10-02T12:48:05","modified_gmt":"2023-10-02T16:48:05","slug":"snp-discovery-services-sanger-sequencing-services","status":"publish","type":"service","link":"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/genotyping\/snp-discovery-services-sanger-sequencing-services\/","title":{"rendered":"SNP Detection Services &#8211; PCR Amplification"},"content":{"rendered":"\n<p>CES offers a Single Nucleotide Polymorphism (SNP) detection service and a complete de novo plasmid sequencing service using primer extension. Please contact the <a href=\"mailto:infoservices@genomequebec.com\" data-type=\"mailto\" data-id=\"mailto:infoservices@genomequebec.com\">Client Management Office<\/a> to find out which option is best suited to your project.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">The next-generation SNP detection\/validation sequencing service includes :<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Design and ordering of primers for target regions<\/li>\n\n\n\n<li>Amplification of target regions with <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/23855-2\/\" target=\"_blank\" data-type=\"URL\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/23855-2\/\" rel=\"noreferrer noopener\">Fluidigm Access Array\u2122<\/a> technology (if applicable)<\/li>\n\n\n\n<li>Sequencing of amplicons using various next-generation technologies (<a href=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/illumina-technologies\/\" data-type=\"URL\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/illumina-technologies\/\" target=\"_blank\" rel=\"noreferrer noopener\">Illumina NovaSeq, NextSeq<\/a> or <a href=\"https:\/\/genomequebec.com\/services-technologiques\/sequencage\/technologie-de-sequencage-en-temps-reel-pacific-biosciences-pacbio-sequel\/\" data-type=\"URL\" data-id=\"https:\/\/genomequebec.com\/services-technologiques\/sequencage\/technologie-de-sequencage-en-temps-reel-pacific-biosciences-pacbio-sequel\/\" target=\"_blank\" rel=\"noreferrer noopener\">PacBio<\/a>)<\/li>\n\n\n\n<li>SNP detection by comparison between between test samples or against a reference sequence<\/li>\n\n\n\n<li>Preparation and dispatch of a variation analysis report, including annotation of the variations found<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">SNP detection\/validation by Sanger sequencing service includes :<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Design and ordering of primers for target regions<\/li>\n\n\n\n<li>PCR amplification of target regions from genomic DNA of test samples<\/li>\n\n\n\n<li>Sequencing of amplicons<\/li>\n\n\n\n<li>SNP detection by comparison between test samples or against a reference sequence<\/li>\n\n\n\n<li>Preparation and submission of a variation analysis report, including annotation of the variations found<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">The de novo sequencing service includes :<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Sanger-type sequencing<\/li>\n\n\n\n<li>Design and ordering of all primers required for complete plasmid sequencing<\/li>\n<\/ul>\n","protected":false},"featured_media":0,"parent":23823,"menu_order":0,"template":"","class_list":["post-23862","service","type-service","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/23862","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/types\/service"}],"up":[{"embeddable":true,"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/23823"}],"wp:attachment":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/media?parent=23862"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}