{"id":23860,"date":"2023-03-27T15:12:16","date_gmt":"2023-03-27T19:12:16","guid":{"rendered":"https:\/\/genomequebec.com\/?post_type=service&#038;p=23860"},"modified":"2025-11-11T12:00:34","modified_gmt":"2025-11-11T17:00:34","slug":"sanger-sequencing-services","status":"publish","type":"service","link":"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/sequencing\/sanger-sequencing-services\/","title":{"rendered":"Sanger Sequencing Services"},"content":{"rendered":"\n<h4 class=\"wp-block-heading\">Sanger DNA sequencing service using Applied Biosystems 3730xl DNA Analyzer technology<\/h4>\n\n\n\n<p>In addition to regular service, CES offers a <em><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\"><span style=\"text-decoration: underline;\">BaseXpress<\/span><\/mark><\/em> sequencing service (certain conditions apply; contact the <a href=\"mailto:infoservices@genomequebec.com\" data-type=\"mailto\" data-id=\"mailto:infoservices@genomequebec.com\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a> for details).<\/p>\n\n\n\n<p>Sanger-type sequencing is widely used to sequence plasmid DNA to verify, for example, the integrity of a gene inserted in an expression vector, or to resequence a target region of a genome and detect the presence of genetic variations (see <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/genotyping\/snp-discovery-services-sanger-sequencing-services\/\" data-type=\"URL\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/genotyping\/snp-discovery-services-sanger-sequencing-services\/\">SNP discovery service<\/a>).<\/p>\n\n\n\n<p>PCR product purification is included with the sequencing service.<\/p>\n\n\n\n<p>Sanger sequencing is most suitable for small to medium range sequencing projects. For large-scale sequencing projects, please contact the <a href=\"mailto:infoservices@genomequebec.com\" data-type=\"mailto\" data-id=\"mailto:infoservices@genomequebec.com\">Client Management Office&nbsp;<\/a>to determine the most appropriate high-throughput sequencing technology..<\/p>\n\n\n\n<p>More details can be found in the <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/user-guides\/#sequencing\" data-type=\"link\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/user-guides\/\">User Guide<\/a>.<\/p>\n\n\n\n<p>The Sanger sequencing methodology is described in the following web site:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.youtube.com\/watch?v=e2G5zx-OJIw\" target=\"_blank\" rel=\"noopener\">How does Sanger Sequencing Work?<\/a><\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h4 class=\"wp-block-heading\">To initiate a sequencing request:<\/h4>\n\n\n\n<p>For information on the prerequisites for sample submission, please consult the <a href=\"https:\/\/genomequebec.com\/wp-content\/uploads\/2023\/06\/RF-Sanger.xls\" data-type=\"URL\" data-id=\"https:\/\/genomequebec.com\/wp-content\/uploads\/2023\/06\/RF-Sanger.xls\" target=\"_blank\" rel=\"noreferrer noopener\">Sanger Request Form<\/a> and send the Excel file as well as a signed copy by the PI of the project to the <a href=\"mailto:infoservices@genomequebec.com\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a>.<\/p>\n\n\n\n<p>More information regarding the requirement are available in the <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/user-guides\/#sequencing\">Sanger User Guide<\/a>. Help documents explaining the steps involved in sample submission, and a tutorial on interpreting results and troubleshooting are also available.<\/p>\n\n\n\n<p><strong>Clients Management Office<\/strong><br><strong>Telephone\u00a0:<\/strong>\u00a0514-345-4738<br><strong>Email\u00a0:<\/strong>\u00a0<a href=\"mailto:infoservices@genomequebec.com\" target=\"_blank\" rel=\"noreferrer noopener\">infoservices@genomequebec.com<\/a><\/p>\n","protected":false},"featured_media":0,"parent":23822,"menu_order":0,"template":"","class_list":["post-23860","service","type-service","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/23860","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/types\/service"}],"up":[{"embeddable":true,"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/23822"}],"wp:attachment":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/media?parent=23860"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}