{"id":23822,"date":"2023-02-15T15:39:25","date_gmt":"2023-02-15T20:39:25","guid":{"rendered":"https:\/\/genomequebec.com\/?post_type=service&#038;p=23822"},"modified":"2026-01-05T13:14:23","modified_gmt":"2026-01-05T18:14:23","slug":"sequencing","status":"publish","type":"service","link":"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/sequencing\/","title":{"rendered":"Sequencing\u00a0"},"content":{"rendered":"\n<h4 class=\"wp-block-heading\">Next Generation Sequencing<\/h4>\n\n\n\n<p>The CES is equipped with the latest next-generation sequencing technologies (Illumina NovaSeq, NextSeq and MiSeq and <a href=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/pacific-biosciences-real-time-sequencing-technology-sequel\/\" data-type=\"URL\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/pacific-biosciences-real-time-sequencing-technology-sequel\/\">PacBio<\/a>). In doing so, the CES has developed expertise in whole genome, exome and targeted region sequencing, epigenome analysis, as well as various applications related to transcriptomics and metagenomics.<\/p>\n\n\n\n<p>The CES provide <strong>library preparation services<\/strong> for a wide variety of applications:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Whole genome sequencing (WGS)<\/li>\n\n\n\n<li>Exome sequencing (WES)<\/li>\n\n\n\n<li>RNA sequencing (RNA-Seq)<\/li>\n\n\n\n<li>Single cell sequencing (scRNA-Seq)<\/li>\n\n\n\n<li>ChIP-Seq<\/li>\n\n\n\n<li>Methylome sequencing (Methyl-Seq)<\/li>\n\n\n\n<li>Targeted region sequencing (<a href=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/23855-2\/\" target=\"_blank\" data-type=\"URL\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/23855-2\/\" rel=\"noreferrer noopener\">Access Array\u2122 from Fluidigm<\/a>)<\/li>\n\n\n\n<li>Metabarcoding service<\/li>\n\n\n\n<li>Amplicon sequencing<\/li>\n\n\n\n<li><a href=\"https:\/\/genomequebec.com\/en\/technological-services\/long-pcr-product-and-whole-plasmid-sequencing-service-using-oxford-nanopore-technology\/\" target=\"_blank\" rel=\"noreferrer noopener\">Long PCR product and whole plasmid sequencing service using Oxford Nanopore technology<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/genomequebec.com\/en\/technological-services\/small-genome-sequencing\/\" target=\"_blank\" rel=\"noreferrer noopener\">Small genome sequencing service using Oxford Nanopore technology<\/a><\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" width=\"864\" height=\"576\" src=\"https:\/\/genomequebec.com\/wp-content\/uploads\/2023\/05\/Salle_de_sequencage_LR-1.jpg\" alt=\"\" class=\"wp-image-26440\" style=\"width:525px;height:349px\" srcset=\"https:\/\/genomequebec.com\/wp-content\/uploads\/2023\/05\/Salle_de_sequencage_LR-1.jpg 864w, https:\/\/genomequebec.com\/wp-content\/uploads\/2023\/05\/Salle_de_sequencage_LR-1-300x200.jpg 300w, https:\/\/genomequebec.com\/wp-content\/uploads\/2023\/05\/Salle_de_sequencage_LR-1-768x512.jpg 768w\" sizes=\"(max-width: 864px) 100vw, 864px\" \/><\/figure>\n\n\n\n<p><strong>Sequencing Technologies:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/sequencing\/illumina-technologies\/\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/sequencing\/illumina-technologies\/\" rel=\"noreferrer noopener\">Illumina NovaSeqX Plus, NextSeq2000 and MiSeq<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/pacific-biosciences-real-time-sequencing-technology-sequel\/\" target=\"_blank\" data-type=\"URL\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/sequencing\/pacific-biosciences-real-time-sequencing-technology-sequel\/\" rel=\"noreferrer noopener\">PacBio Sequel II<\/a><\/li>\n<\/ul>\n\n\n\n<p>Sequencing-ready libraries can also be sent for Illumina sequencing.<\/p>\n\n\n\n<p>Reads are delivered in FASTQ format, and BAM files are available for human, rat and mouse samples. Sequences are downloaded directly from <a href=\"https:\/\/genomequebec.com\/en\/nanuq\/\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/genomequebec.com\/en\/nanuq\/\" rel=\"noreferrer noopener\">Nanuq<\/a>.<\/p>\n\n\n\n<p>All services include a free initial consultation to discuss experimental design and appropriate technologies for each project. Contact the <a href=\"mailto:infoservices@genomequebec.com\" target=\"_blank\" data-type=\"mailto\" data-id=\"mailto:infoservices@genomequebec.com\" rel=\"noreferrer noopener\">Client Management Office<\/a>.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Sanger sequencing<\/h4>\n\n\n\n<p>CES also offers Sanger-type DNA sequencing services. With its two Applied Biosystems 3730xl DNA Analyzer instruments and an annual capacity in excess of 1 million high-quality reactions, CES can rapidly sequence PCR products, plasmids, phages and BAC ends, as well as carry out your SNP detection projects. The CES also offers a sequencing service: BaseXpress.<\/p>\n\n\n\n<p>Services provided:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/sequencing\/sanger-sequencing-services\/\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/sequencing\/sanger-sequencing-services\/\" rel=\"noreferrer noopener\">Sanger Sequencing<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/genotyping\/snp-discovery-services-sanger-sequencing-services\/\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/genotyping\/snp-discovery-services-sanger-sequencing-services\/\" rel=\"noreferrer noopener\">SNP Discovery &#8211; PCR amplifications<\/a><\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/genomequebec.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/user-guides\/#sequencing\">User Guides<\/a><\/div>\n<\/div>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"576\" height=\"864\" src=\"https:\/\/genomequebec.com\/wp-content\/uploads\/2023\/05\/Sequencage_Sanger_LR.jpg\" alt=\"\" class=\"wp-image-23750\" srcset=\"https:\/\/genomequebec.com\/wp-content\/uploads\/2023\/05\/Sequencage_Sanger_LR.jpg 576w, https:\/\/genomequebec.com\/wp-content\/uploads\/2023\/05\/Sequencage_Sanger_LR-200x300.jpg 200w\" sizes=\"(max-width: 576px) 100vw, 576px\" \/><\/figure>\n","protected":false},"featured_media":334,"parent":26647,"menu_order":0,"template":"","class_list":["post-23822","service","type-service","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/23822","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/types\/service"}],"up":[{"embeddable":true,"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/service\/26647"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/media\/334"}],"wp:attachment":[{"href":"https:\/\/genomequebec.com\/en\/wp-json\/wp\/v2\/media?parent=23822"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}