Cellular metabolism is the set of chemical reactions that maintain life. The field of metabolomics is dedicated to studying and measuring these essential biological processes through information-rich technologies, such as mass spectrometry. While protocols that measure gene expression or protein levels have been adapted to single cell analysis, researchers currently can only measure metabolites within bulk cell populations or tissues. Indeed, single cell omics technologies have revolutionized our understanding of complex, heterogeneous diseases such as cancer. However, DNA sequence and RNA transcript levels do not correlate directly with levels of proteins or metabolites. To understand complex phenotypes at the level of an individual cell, single cell metabolomics methods are required. The overall objective of this project is to develop a workflow for the measurement of targeted metabolites at a single cell resolution in cancer cells.
Lead Genome Centre: Génome Québec
User :
| Gaëlle | Bridon | Agilent Technologies |