Campylobacter is a major foodborne pathogen worldwide, and highly associated with agroecosystem and food processing environment. Campylobacteriosis is the most frequently reported bacterial illness in Canada, outnumbering the reported cases of Listeria monocytogenes, Shiga toxigenic Escherichia coli and Salmonella infections combined. The contamination of this pathogenic microbe has a significant economic impact on the poultry industry. Current methods for the detection of Campylobacter in poultry products have various disadvantages, such as time consuming and labor intensive.
In this project, we aim to develop an isothermal amplification-CRISPR/Cas12a based lateral flow assay for rapid detection of Campylobacter in poultry products. CRISPR/Cas systems have been extensively used for gene editing and transcriptional regulation and show great promise in molecular diagnosis. This new assay will be more rapid, accurate, sensitive and affordable than the current commercial ones, and brings benefits to the government agencies and industry stakeholders performing regular testing of Campylobacter contamination.
Genome Centre: Génome Québec
Sylvain Fournaise – Olymel